The functional significance of this tomato ERF family member was first addressed by attempting to alter its expression using antisense or over-expression strategies. A chimeric TALE-SRDX repressor can be used to repress the transcription of the transgene RD29A::LUC and endogenous gene RD29A in A. Values are means ± SD of three biological replicates. Here, transcriptional activator-like effectors (TALEs), containing a modular DNA-binding domain, can be used to generate chimeric transcriptional activators or repressors. Our data suggest that TALEs can be used to generate chimeric repressors to specifically repress the transcription of genes of interest in plants. B3-SRDX dominant repressor lines ( SR1, SR2 and SR3) and control wild-type (WT) fruit at the 3 d post-breaker stage. Genome wide expression profiling showed that the chimeric repressor also inhibited the expression of several other genes that contain the designer TALE-target sequence in their promoters. SRDX protein efficiently repressed the transcription of the RD29A::LUC transgene and endogenous RD29A gene in Arabidopsis. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Chimeric repressor of PtSND2 severely affects wood formation in transgenic Populus. The dHax3.SRDX protein efficiently repressed the transcription of the RD29A::LUC transgene and endogenous RD29A gene in Arabidopsis. Abbreviation / Long Form : SRDX / SUPERMAN REPRESSION DOMAIN X Related PubMed/MEDLINE Total Number of Papers: 2 Entries Per Page per page Page Control Page: of. Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter.

Srdx repressor